![]() ![]() Thus “housekeeping genes” are frequently chosen for use as loading controls. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. The expression levels of the loading control should not vary between the different sample lanes. They are used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. There is a wide range from mouse monoclonals to goat polyclonal antibodies, for a variety of applications and reactivities.Loading controls are essential for proper interpretation of western blots. Negative control - Secondary onlyĮxplore St John's Laboratory beta actin antibodies below. Antigen retrieval - Sodium Citrate pH6 (>98C, 20min). Anti-Beta actin antibody was applied at 1:200 (4C,overnight). STJ91464- Immunohistochemical analysis of human colon tissue. ![]() STJ96930 - Immunohistochemical analysis of rat testis tissue. St John’s Laboratory has many different Beta Actin antibodies which have been used in various techniques. ![]() Researchers can take advantage of antibody binding not just in western blotting, but Beta Actin antibodies can be used to detect the presence of the protein in procedures such as: immunohistochemical analysis and immunofluorescence. beta actin antibodies are created by raising B lymphocytes in the presence of beta actin, the antibodies will form, the peptide region specific to beta actin antigen epitope. In order to detect proteins such as beta actin during a western blot, antibodies are used as their targeting and binding abilities can be utilised to visually assist researchers in the indication of the presence or absence of protein expression. If you’d like to read up more about other loading controls, click here to check them out. During a western blot beta actin is used as, and is one of the most commonly used loading controls.Ī loading control is used to provide a more reliable interpretation of western blot data, as proteins like beta actin are generally unaffected by experimental conditions.īeta Actin can be used to normalize the levels of protein detected by acting as a control level, allocating it the all-important role of confirming that protein loading is the same across the gel. Making it quite the busy bee.ta protein.īeta actin has a molecular weight of 42kD, because of its high abundance and universal expression across various cell types, beta actin is researchers go to protein when conducting experiments involving western blotting. Beta actin specifically plays a crucial role in other relating functions like cell migration, involved in pathways at the blood-brain barrier, cell division, muscle contraction, cell mobility, secretion, phagocytosis and gene expression. It is a major cytoskeletal filament protein found in eukaryotes, prokaryotes and fungi. It is a highly conserved molecule and is involved in more protein-protein interactions than any known protein!īeta Actin is one of six Isomers of the actin protein. Out of all the thousands and thousands of known proteins that exist within the human genome, actin is the most abundant and unchanged filament protein found in almost every cell type. ![]()
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